Devices and methods for sample analysis with serial dilution

A method comprising following steps:

• (a) using a fluidic cartridge to receive a sample, the fluidic cartridge comprising:
• a first chamber to receive a sample mixture 1;
• and
• a metering chamber for metering a first portion of the sample mixture 1, the metering chamber being connected to the first chamber;
• and
• (b) placing the fluidic cartridge into a reader instrument to perform sample analysis of the sample,
• wherein:
• a driving mechanism is applied to the fluidic cartridge to meter the first portion of the sample mixture 1 in the metering chamber;
• and
• the driving mechanism is stopped after the sample mixture 1 contacting a hydrophilic surface outside the first chamber, the hydrophilic surface being a part of a surface of the metering chamber.

A device comprising a fluidic cartridge, wherein the fluidic cartridge comprises:

• a first chamber to receive a sample mixture 1;
• a metering chamber for metering a first portion of the sample mixture 1, the metering chamber being connected to the first chamber;
• and
• a reader instrument for receiving the device and performing a sample analysis,
• wherein:
• a driving mechanism is configured to be applied to the fluidic cartridge to meter the first portion of the sample mixture 1 in the metering chamber;
• the driving mechanism is configured to drive the sample mixture 1 to contact a hydrophilic surface outside the first chamber, the hydrophilic surface being a part of a surface of the metering chamber;
• and
• the driving mechanism is configured to be stopped before the sample mixture 1 reaches a capillary valve in the metering chamber.