Method for Capturing Ancient DNA of Wooden Cultural Relic

A method for capturing an ancient DNA of a wooden cultural relic, comprising:

• step 100:
• cutting a wood sample with a volume V from the wooden cultural relic, and removing an exogenous DNA contamination from the wood sample, wherein
• a method for removing the exogenous DNA contamination of the wood sample sequentially comprises, an ultraviolet irradiation, an outer surface removal, a soaking, and a washing,
• the ultraviolet irradiation is kept for 5-48 h under a radiation of 10-50 W,
• the outer surface removal is carried out by cutting off an outer surface of the wood sample by 1-4 mm using a sterile scalpel,
• the soaking is to soak the wood sample in a sodium hypochlorite solution containing 10.01-0.1.0% Tween-20 with a mass fraction of 1-5% for 1-10 min, the washing is carried out using a double distilled water for 1-3 times, each time for 1-5 min;
• step 200:
• grinding the wood sample into a. powder sample at a low temperature;
• step 300:
• rinsing the powder sample 2-3 times to obtain a rinsed powder sample, wherein a rinsing solution CB for rinsing the powder sample includes 10-50 mM Tris-HCl at pH=8.0, 5-20 mM EDTA at pH=8.0, 5-10% PVP-40, 5% glycerol, 5-10% PEG8000, 300-500 sorbitol, and 50 mM DTT;
• step 400:
• sequentially carrying out a cell lysis, a DNA purification, and a concentration on the rinsed powder sample to obtain and collect a sample genomic DNA;
• step 500:
• constructing a DNA library acid designing an RNA probe based on the sample genomic DNA, and then hybridizing the RNA probe with the DNA library to form an RNA-DNA complex solution;
• step 600:
• capturing an RNA-DNA complex from the RNA-DNA complex solution using a streptavidin-labeled magnetic bead;
• step 700:
• washing the RNA-DNA complex to obtain a washed RNA-DNA complex, and then dissolving the washed RNA-DNA complex to obtain a target DNA product;
• and
• step 800:
• carrying out a PCR amplification on the target DNA product, and constructing a target DNA library for a high-throughput sequencing.