Back to results
US 2023/0265491 A1Published application
Spatial transcriptomic transfer modes
Assignee10x Genomics, Inc.
InventorsAugusto Manuel Tentori, Hanyoup KIM, Rajiv Bharadwaj
Life of this patent
Estimating the term…
Abstract
Provided herein are methods and systems for determining the location of one or more analytes in a biological sample with electrophoresis analyte transfer modes.
Claims21
1-156. (canceled)
A method for determining a location of one or more analytes in a biological sample, the method comprising:
- (a) providing an array comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises:
- (i) a spatial barcode and (ii) a capture domain;
- (b) providing one or more semi-porous materials, wherein the one or more semi-porous materials are disposed between a biological sample and the array;
- (c) applying an electric field to the biological sample, the one or more semi-porous materials, and the array, wherein the electric field promotes the migration of the one or more analytes in the direction of the array, wherein the capture domain of the capture probe specifically binds to a first analyte of the one or more analytes, wherein the first analyte is a nucleic acid;
- and
- (d) determining (i) the sequence of the spatial barcode, or a complement thereof, and (ii) all or a portion of the sequence of the nucleic acid, or a complement thereof, and using the sequences of (i) and (ii) to determine the location of the first analyte in the biological sample.
3 more elements
Dependencies are inferred from the claim text.