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AssigneeLallemand Hungary Liquidity Management LLC
InventorsCharles F. Rice, Ryan Skinner, Trisha Barrett, Aaron Argyros
Estimating the term…
The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).
A method of increasing the production of a fermentation product during a fermentation, said method comprising contacting a medium with (i) a recombinant yeast host cell comprising a first genetic modification for expressing a heterologous STL1 protein and (ii) a trehalase, wherein the production of the fermentation product is increased when compared to a control method with a control yeast host cell lacking the first genetic modification.
A combination comprising (i) a recombinant yeast host cell comprising a first genetic modification for expressing a heterologous STL1 protein and (ii) a trehalase.
Dependencies are inferred from the claim text.