Cannabis with altered cannabinoid content
AssigneeBetterseeds Ltd
InventorsTal SHERMAN, Ido MARGALIT, Shira COREM
Life of this patent
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Abstract
Provided is a Cannabis plant exhibiting altered tetrahydrocannabinolic acid (THCA) and/or cannabidiolic acid (CBDA) content. The plant includes a modified genomic locus involved in tetrahydrocannabinolic acid synthase (THCAS) and/or cannabidiolic acid synthase (CBDAS) gene expression, the genomic locus includes at least one targeted nucleotide modification within a regulatory region modulating the expression of at least one allele of the THCAS and/or CBDAS genes. Further provided are methods for producing the aforementioned Cannabis plant.
Claims21
-90. (canceled)
A method for altering tetrahydrocannabinolic acid synthase (THCAS) or cannabidiolic acid synthase (CBDAS) gene expression in a Cannabis plant or a cell thereof, the method comprising steps of introducing one or more nucleotide modifications through targeted genome modification at a regulatory region modulating the expression of said THCAS or CBDAS gene.
A guide RNA (gRNA) sequence that targets a tetrahydrocannabinolic acid synthase (THCAS) or cannabidiolic acid synthase (CBDAS) genomic locus of a plant cell, wherein the gRNA is complementary to at least one target sequence of a regulatory region within said genomic locus, said regulatory region operably linked to the expression of a THCAS or CBDAS gene allele.
Use of a nucleotide sequence having at least 70% sequence identity to a nucleotide sequence as set forth in at least one of SEQ ID NO:
- 7-91, SEQ ID NO:
- 92-176, SEQ ID NO:
- 177-278, SEQ ID NO:
- 279-419, SEQ ID NO:
- 420-560, SEQ ID NO:
- 561-681, SEQ ID NO:
- 682-794, SEQ ID NO:
- 795-895, SEQ ID NO:
- 896-1016, SEQ ID NO:
- 1017-1149, for targeted genome modification of pFNTHCAS-1, pFNTHCAS-2, pPKTHCAS-1, pPKTHCAS-2, pPKTHCAS-3, pPKTHCAS-4, pCBDAS2, pFNCBDAS, pPKCBDAS and pPKCBDAS1 gene, respectively;
- and/or
- use of a nucleotide sequence having at least 70% sequence identity to a nucleotide sequence as set forth in at least one of SEQ ID NO:
- 7-681 and any combination thereof for reducing THCA content in a Cannabis plant or a cell thereof;
- and/or
- use of a nucleotide sequence having at least 70% sequence identity to a nucleotide sequence as set forth in at least one of SEQ ID NO:
- 682-1149 and any combination thereof for reducing CBDA content in a Cannabis plant or a cell thereof.
Dependencies are inferred from the claim text.