Reduction of fatty acid retinyl ester formation
AssigneeDsm IP Assets B.V.
InventorsPeter Louis HOUSTON, Valmik Kanubhai VYAS, René Marcel DE JONG, Anna SYMBOR-NAGRABSKA, Ethan LAM
Life of this patent
Estimating the term…
Abstract
The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, growing on triglyceride oils, such as e.g. vegetable oil, wherein the host cell exhibits modified lipase activity in such a way that conversion of retinol into fatty acid retinyl esters (FAREs) is reduced or abolished. Such process is especially useful in a biotechnological process for production of vitamin A.
Claims10
A retinoid-producing host cell capable of retinyl acetate formation, particularly retinyl acetate-producing host cell, such as a fungal host cell, preferably oleaginous yeast cell such as e.g. Yarrowia, comprising:
- (1) one or more genetic modification(s), such as reduction or abolishment, preferably abolishment, of endogenous genes encoding enzymes involved in pre-digestion of vegetable oil into glycerol and fatty acids, preferably endogenous enzymes belonging to EC class 3.1.1.-, more preferably genes encoding endogenous enzymes with esterase or lipase activity;
- and
- (2) addition of a heterologous enzyme with lipase activity, preferably enzymes belonging to EC class 3.1.1.-, particularly enzymes with fungal lipase activity, more preferably lipase activity from Candida species, most preferably Candida rugosa lipase activity.
Dependencies are inferred from the claim text.