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AssigneeMeiragtx UK II Limited
InventorsXuecui Guo, Alexandria Forbes, Kevin G. Liu, Ji-In Kim
Estimating the term…
The present disclosure relates to oligonucleotide aptamers that bind to certain small molecules including thiamine analogs and derivatives and methods of generating aptamers that bind to the small molecules. Also contemplated are riboswitches and polynucleotide cassettes comprising the aptamers disclosed herein. Further provided are methods of using said aptamers, riboswitches, and/or polynucleotide for the regulation of target genes, including therapeutic genes. Also provided herein are small molecules that are modulators of target gene expression where the target gene contains a riboswitch comprising an aptamer described herein.
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A polynucleotide cassette for regulating the expression of a target gene, wherein the polynucleotide cassette comprises a sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises a sequence that is at least 95% identical to a sequence selected from the group consisting of SEQ ID NOs:7-36.
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises
A nucleic acid sequence encoding an aptamer that binds to a small molecule, wherein the aptamer encoding sequence comprises a sequence that is at least 95% identical to a sequence selected from the group consisting of SEQ ID NOs:7-36.
The polynucleotide cassette of claim [0040], wherein the riboswitch comprises the sequence of SEQ ID NO:37.
The polynucleotide cassette of claim [0040], wherein the polynucleotide cassette is located in the protein coding sequence of the target gene.
The polynucleotide cassette of claim [0040], wherein the polynucleotide cassette is located in an untranslated region of the target gene or in an intron of the target gene.
A method for identifying an aptamer that modulates target gene expression in response to a compound of interest, the method comprising the steps of:
Dependencies are inferred from the claim text.