Back to results
US 2023/0265408 A1Published application
Protease variants with improved solubility
AssigneeNovozymes A/S
InventorsRolf Thomas Lenhard, Esben Peter Friis, Poul Erik Pedersen, Rasmus Lund Andersen
Life of this patent
Estimating the term…
Abstract
The present invention relates to protease variants, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising said polynucleotides, host cells expressing said variants, methods of obtaining the variants, detergent compositions comprising said variants, and use of said variants or said detergent compositions.
Claims24
A protease variant of a parent protease, wherein the variant has a sequence identity of at least at least 80%, but less than 100%, to SEQ ID NO:1;
- wherein the variant comprises a first substitution selected from the group consisting of X215K, X215R, X215Q, X125N, X215S, and X215T;
- wherein the variant comprises at least three further alterations, preferably substitutions, selected from the group consisting of X3T (e.g., S3T), X4I (e.g., V4I), X9E (e.g., S9E), I35ID, X43R (e.g., N43R), X76D (e.g., N76D), X99D (e.g., S99D, X99F (e.g., S99F), X101E (e.g., S101E), X101L (e.g., S101L), X103A (e.g., S103A), X103T (e.g., S103T), X104I (e.g., V104I), X120D (e.g., H120D), X160S (e.g., G160S), X195E (e.g., G195E), X205I (e.g., V205I), X206L (e.g., Q206L), X209W (e.g., Y209W), X235L (e.g., K235L), X259D (e.g., S259D), X261W (e.g., N261W), and X262E (e.g., L262E);
- wherein the variant has protease activity;
- and
- wherein position numbers are based on the numbering of SEQ ID NO:2.
2 more elements
(canceled)
Dependencies are inferred from the claim text.