T cell-antigen coupler with y182t mutation and methods of uses thereof

An engineered γδ T cell comprising an expression vector comprising a nucleic acid encoding a T Cell-Antigen Coupler (TAC) polypeptide, the TAC polypeptide comprising:

• (a) an antigen-binding antibody fragment or DARPin that selectively binds a target antigen;
• (b) a humanized UCHT1 (huUCHT1) polypeptide comprising the amino acid sequence of SEQ ID NO:
• 28;
• and
• (c) a T cell co-receptor domain polypeptide comprising a CD4 cytosolic domain and a CD4 transmembrane domain,
• wherein the TAC polypeptide is expressed by the engineered γδ T cell.