Monoclonal antibody for spike protein of middle east respiratory syndrome coronavirus, and use thereof

A method for detecting Middle East Respiratory Syndrome coronavirus (MERS-CoV) antigen, said method comprising:

• performing an antigen-antibody reaction using a monoclonal antibody, the monoclonal antibody having binding specificity to Middle East Respiratory Syndrome coronavirus (MERS-CoV) spike protein, the monoclonal antibody comprising:
• human immunoglobulin heavy chain constant region and human immunoglobulin light chain constant region, and
• a combination of heavy chain variable region (VH) and light chain variable region (VL) selected from the group consisting of:
• VH according to SEQ ID NOs:
• 1 and VL according to SEQ ID NOs 2;
• VH according to SEQ ID NOs:
• 3 and VL according to SEQ ID NOs 4;
• VH according to SEQ ID NOs:
• 9 and VL according to SEQ ID NOs 10;
• VH according to SEQ ID NOs:
• 11 and VL according to SEQ ID NOs 12;
• VH according to SEQ ID NOs:
• 19 and VL according to SEQ ID NOs 20;
• and
• VH according to SEQ ID NOs:
• 21 and VL according to SEQ ID NOs 22.

A method, comprising:

• contacting Middle East Respiratory Syndrome coronavirus (MERS-CoV) with a monoclonal antibody, the monoclonal antibody having binding specificity to MERS-CoV spike protein, the monoclonal antibody comprising:
• human immunoglobulin heavy chain constant region and human immunoglobulin light chain constant region, and
• a combination of heavy chain variable region (VH) and light chain variable region (VL) selected from the group consisting of:
• VH having an amino acid sequence according to SEQ ID NOs:
• 1 and VL having an amino acid sequence according to SEQ ID NOs 2;
• VH having an amino acid sequence according to SEQ ID NOs:
• 3 and VL having an amino acid sequence according to SEQ ID NOs 4;
• VH having an amino acid sequence according to SEQ ID NOs:
• 9 and VL having an amino acid sequence according to SEQ ID NOs 10;
• VH having an amino acid sequence according to SEQ ID NOs:
• 11 and VL having an amino acid sequence according to SEQ ID NOs 12;
• VH having an amino acid sequence according to SEQ ID NOs:
• 19 and VL having an amino acid sequence according to SEQ ID NOs 20;
• and
• VH having an amino acid sequence according to SEQ ID NOs:
• 21 and VL having an amino acid sequence according to SEQ ID NOs 22.