QTY FC Fusion Water Soluble GPCR Proteins
AssigneesMassachusetts Institute of Technology, Avalon GloboCare Corp.
InventorsShuguang ZHANG, David JIN, Rui QING, Uwe SLEYTR
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Abstract
The present invention is directed to Fc fusion water soluble GPCR (G-protein coupled receptor) proteins with QTY membrane regions, wherein the amino acids Q (Glutamine), T (Threonine), and Y (Tyrosine), together with other amino acids can form an alpha helical domain that mimics a transmembrane region, and the QTY code conversions render the alpha-helical segment water-soluble. Further disclosed are methods for the preparation QTY Fc receptor and methods of use thereof.
Claims40
A Fc fusion QTY protein comprising an Fc domain and one or more QTY GPCR domains.
The Fc fusion QTY GPCR protein of any one of the preceding claims, wherein the QTY GPCR domain comprises each extracellular domain of a human chemokine receptor.
The Fc fusion QTY GPCR protein of any one of the preceding claims wherein each transmembrane region has the amino acid sequence of a cytokine receptor transmembrane domain wherein a plurality of hydrophobic amino acids is replaced with a Q, Tor Y.
The Fc fusion QTY GPCR protein of any one of the preceding claims wherein the protein binds a chemokine.
The Fc fusion QTY GPCR protein of any one of the preceding claims wherein the Fc domain comprises a human immunoglobulin constant region.
The Fc fusion QTY GPCR protein of any one of the preceding claims wherein the Fc domain comprises an IgG constant domain.
The Fc fusion QTY GPCR protein of any one of the preceding claims wherein the Fc Domain is fused to the QTY GPCR domain via a hinge region.
The Fc fusion QTY GPCR protein of any one of the preceding claims wherein comprising two identical QTY GPCR domains.
Dependencies are inferred from the claim text.