Mhc multimer expression constructs and uses thereof
AssigneeRepertoire Immune Medicines, Inc.
InventorsJoanna Swain, Michael Birnbaum
Life of this patent
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Abstract
MHC multimer expression constructs are provided that contiguously encode an MHC-binding peptide, MHC molecule chains and a multimerization domain in the construct such that expression in a host cell results in production of peptide-loaded MHC (pMHC) multimers by the host cell. The multimers can further comprise oligonucleotide barcodes. Peptide exchange can be performed with a plurality of pMHC multimers to create pMHC multimer libraries. Methods of making and using the pMHC multimers and libraries are also provided. Peptide-loaded MHC Class I and MHC Class II multimers, and libraries thereof, are provided.
Claims78
A method of producing a Major Histocompatibility Complex (MHC) multimer, the method comprising:
- (a) providing an MHC multimer expression construct comprising a nucleic acid encoding (i) an MHC-binding peptide operatively linked to a cleavage site;
- (ii) a first MHC subunit;
- (iii) a second MHC subunit;
- and (iv) a multimerization domain;
- (b) introducing the MHC multimer expression construct into a host cell;
- and
- (c) expressing the MHC multimer in the host cell.
An isolated Major Histocompatibility Complex (MHC) multimer expression construct, the construct comprising a nucleic acid encoding (i) an MHC-binding peptide operatively linked to a cleavage site;
- (ii) a first MHC subunit;
- (iii) a second MHC subunit;
- and (iv) a multimerization domain.
Dependencies are inferred from the claim text.