Cell culture solution container and dispenser

A method of manufacturing a cell culture solution container, comprising:

• providing a rigid first end;
• providing a rigid second end;
• providing a deformable middle portion;
• attaching the deformable middle portion to the first end and second end, wherein the first end, second end, and middle portion enclosing a region having a variable volume;
• providing an inlet;
• fluidly coupling the inlet to the region;
• providing an outlet;
• fluidly coupling the outlet to the region;
• and
• loading cell culture solution powder in the region.

A method of creating culture solution, comprising:

• receiving, at a dispenser, a first container in a collapsed configuration;
• expanding the first container to an expanded configuration;
• while the first container is expanding or the first container is in the expanded configuration:
• injecting water into the first container;
• driving a stirrer in the first container;
• and
• mixing, with the stirrer, a powder and the water in the first container to create the culture solution;
• and
• dispensing the culture solution into a second container, wherein the first container comprises:
• a rigid first end;
• a rigid second end;
• a deformable middle portion attached to the first end and second end and enclosing, with the first and second ends, a region having a variable volume;
• the powder in the region;
• an inlet fluidly coupled to the region;
• and
• an outlet fluidly coupled to the region.