Methods and compositions for treating coronaviral infections
AssigneeYeda Research and Development Co. Ltd.
InventorsGideon SCHREIBER, Jiri ZAHRADNIK, Yinon RUDICH
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Abstract
A polypeptide comprising an amino acid sequence of SARS CoV-2 receptor-binding domain (RBD) is disclosed, wherein said amino acid sequence comprises a modification at position 358 and at least two additional modifications at two positions selected from the group consisting of 484, 498 and 501, wherein the polypeptide binds soluble, monomeric angiotensin-converting enzyme 2 (ACE2) receptor. Uses thereof are further disclosed.
Claims20
A polypeptide comprising an amino acid sequence of SARS CoV-2 receptor-binding domain (RBD), wherein said amino acid sequence comprises a modification at position 358 and at least two additional modifications at two positions selected from the group consisting of 484, 498 and 501, wherein the numbering of the positions of the modifications is according to UniProtKB - P0DTC2 (SEQ ID NO:
- 37), wherein said polypeptide binds soluble, monomeric angiotensin-converting enzyme 2 (ACE2) receptor when expressed on the surface of yeast cells with at least 50 fold higher affinity than the wild-type RBD having an amino acid sequence as set forth in SEQ ID NO:
- 45, when assayed under identical conditions, wherein said polypeptide comprises at least 170 amino acids of said RBD.
An isolated polypeptide comprising an amino acid sequence at least 99 % identical to SEQ ID NO:
- 38, 39 or 40.
A dimer comprising two monomers linked by a linker, wherein each of said two monomers comprises an amino acid sequence encoding SARS-CoV-2 receptor-binding domain (RBD), wherein each of said monomers binds soluble, monomeric angiotensin-converting enzyme 2 receptor when expressed on the surface of yeast cells with at least 50 fold higher affinity than the wild-type RBD having an amino acid sequence as set forth in SEQ ID NO:
- 45, when assayed under identical conditions.
Dependencies are inferred from the claim text.