Low fiber pennycress meal, seeds, and methods of making
AssigneesCovercress Inc., The Board of Trustees of Illinois State University, Regents of the University of Minnesota
InventorsTim Ulmasov, Gary Hartnell, John C. Sedbrook, Michael David Marks, Ratan Chopra, Maliheh Esfahanian
Life of this patent
Estimating the term…
Abstract
Pennycress seed, seed lots, and seed meal having reduced fiber content and improved suitability for use in producing animal feed are provided.
Claims22
1-80. (canceled)
Pennycress seed meal comprising an acid detergent fiber (ADF) content of 7% to 25% by dry weight, wherein the seed meal is defatted, and wherein said meal comprises a detectable amount of a polynucleotide comprising:
- (i) at least one loss-of-function mutation in an endogenous wild-type pennycress gene comprising the polynucleotide sequence of SEQ ID NO:
- 56 or SEQ ID NO:
- 59;
- or (ii) at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene having at least 95% sequence identity to SEQ ID NO:
- 56 or SEQ ID NO:
- 59.
Pennycress seed meal comprising an acid detergent fiber (ADF) content of 5% to 20% by dry weight, wherein the seed meal is non-defatted, and wherein said meal comprises a detectable amount of a polynucleotide comprising:
- (i) at least one loss-of-function mutation in an endogenous wild-type pennycress gene comprising the polynucleotide sequence of SEQ ID NO:
- 56 or SEQ ID NO:
- 59;
- or (ii) at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene having at least 95% sequence identity to SEQ ID NO:
- 56 or SEQ ID NO:
- 59.
A seed lot comprising a population of pennycress seeds that comprise an acid detergent fiber (ADF) content of 5% to 20% by dry weight, wherein the population comprises at least 10 seeds comprising said ADF content and wherein said population of pennycress seeds comprise:
- (i) seeds having at least one loss-of-function mutation in an endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO:
- 55 or SEQ ID NO:
- 58;
- (ii) seeds having at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:
- 55 or SEQ ID NO:
- 58;
- (iii) seeds having at least one transgene that suppresses expression of an endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO:
- 55 or SEQ ID NO:58;
- or (iv) seeds having at least one transgene that suppresses expression of an allelic variant of the endogenous wild-type pennycress gene encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:
- 55 or SEQ ID NO:
- 58.
Dependencies are inferred from the claim text.