Biomarkers Predictive of Atopic Dermatitis

A method for evaluating the efficacy of a skin treatment regimen, ingredient and/or composition to treat atopic dermatitis, comprising:

• (a) measuring the level of hBD1, IL1RA, IL36g and 5100A8/9 on an area of skin prior to application of the skin treatment regimen, ingredient and/or composition;
• (b) applying the skin treatment regimen, ingredient and/or composition to the area of skin for a period of time;
• (c) measuring the level of hBD1, IL1RA, IL36g and 5100A8/9 after the skin treatment regimen, ingredient and/or composition application on the area of skin;
• wherein the skin treatment regimen, ingredient and/or composition is beneficial to the skin if the level of the hBD1, IL1RA, IL36g and 5100A8/9 is less than or equal to the no treatment control.

A method of predicting a propensity of an individual to develop atopic dermatitis, comprising:

• a) observing the expression of at least one skin microbe species selected from those in Table 2 on a skin area;
• b) comparing said expression to a determined standard, wherein said determined standard is ascertained by measuring a level of said at least one skin microbe in a subject or pool of subjects who have demonstrated an absence of atopic dermatitis;
• and
• c) determining the propensity of an individual to develop atopic dermatitis,
• wherein an increase in the expression as compared to said determined standard indicates propensity of said individual to develop atopic dermatitis.

A method of predicting a propensity of an individual to develop atopic dermatitis, comprising:

• a) observing total amino acid content on a skin area;
• b) comparing said total amino acid content to a determined standard, wherein said determined standard is ascertained by measuring a level of said total amino acid content in a subject or pool of subjects who have demonstrated an absence of atopic dermatitis;
• and
• c) determining the propensity of an individual to develop atopic dermatitis,
• wherein a decrease in total amino acid content as compared to said determined standard indicates propensity of said individual to develop atopic dermatitis.

A method of predicting a propensity of an individual to develop atopic dermatitis, comprising:

• a) observing two or more parameters selected from the group consisting of (i) the expression of a biomarker selected from hBD1, IL1RA, IL36g and S100A8/9 or a combination thereof on a skin area;
• (ii) the expression of at least one skin microbe species selected from those in Table 2 on a skin area;
• and (iii) the total amino acid content on a skin area;
• b) comparing said two or more parameters to a determined standard for each of said two or more parameters, wherein each of said determined standards is ascertained by measuring a level of said respective parameter in a subject or pool of subjects who have demonstrated an absence of atopic dermatitis;
• and
• c) determining the propensity of an individual to develop atopic dermatitis,
• wherein a change in said parameter as compared to said determined standard indicates propensity of said individual to develop atopic dermatitis.