Biodegradable biochemical sensor for determining the presence and/or the level of pesticides or endocrine disruptors: method and composition

A composition to detect the presence or the absence, and/or to quantify the amount of at least one target analyte in a sample said composition comprising biochemical elements forming a biochemical network encapsulated or not in one or in a set of vesicles permeable or not to the target analyte, said biochemical network comprising as biochemical element at least one enzyme selected from the group of:

• glycine/glyphosate oxidase (EC 1.4.3.19), preferably the native (wild type/WT) glycine/glyphosate oxidase which can be obtained as recombinant protein, or homolog sequence thereof having at least 70% identity with the WT protein sequence and exhibiting glycine/glyphosate oxidase activity;
• 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19);
• the glycine oxidase (GO) from the marine bacteria Bacillus licheniformis ((BliGO) which has been cloned and which shows at least 62% similarity to the standard GO from Bacillus subtilis, or homolog BliGO sequence thereof having at least 70% identity with the BliGO WT protein sequence and exhibiting GO activity;
• the mutated glycine oxidase (GO) from the marine bacteria Bacillus licheniformis ((BliGO)_SCF4 genetically modified and containing 6 single amino-acids mutation compared to the wild type version BliGO-WT or the BliGO-Mut;
• a tagged glyphosate oxidase enzyme, preferably with a tag selected from the group consisting of maltose-binding protein (MBP), Chitin Binding Protein (CBP), glutathione S-transferase (GST), thioredoxin (TRX), NUS A, ubiquitin (Ub), and SUMO (small ubiquitin-related modifier) tags, preferably SUMO and GST tags;
• and
• the GST-BliGO (native/WT) having the DNA sequence SEQ ID NO:5 or the amino acids sequence SEQ ID NO:6;
• the SUMO-BliGO (native/WT) having the DNA sequence SEQ ID NO:9 or the amino acids sequence SEQ ID NO:10;
• the GST-BliGO-Mut having the DNA sequence SEQ ID NO:7 or the amino acids sequence SEQ ID NO:8 and the SUMO-BliGO-Mut having the DNA sequence SEQ ID NO:11 or the amino acids sequence SEQ ID NO:12 and
• homolog tagged BliGO sequences thereof as defined above wherein the BliGO sequence exhibits at least 70%.