Genetically engineered rhodopseudomonas palustris
AssigneeWashington University
InventorsArpita Bose, Wei Bai
Life of this patent
Estimating the term…
Abstract
Among the various aspects of the present disclosure is the provision of a genetically engineered transgenic microorganisms Rhodopseudomonas palustris and methods of making and using the same.
Claims21
1-30. (canceled)
A plasmid-based method for integrating a target gene into a genome of Rhodopseudomonas palustris TIE-1 (TIE-1), the method comprising:
- introducing an attB site into the genome of TIE-1;
- providing a suicide plasmid including an attP site and a target gene;
- providing a plasmid including a φC31 integrase;
- and
- expressing the φC31 integrase in the presence of the attB site and the attP site such that the target gene is integrated into the genome.
A genome-based method for integrating a target gene into a genome of Rhodopseudomonas palustris TIE-1 (TIE-1), the method comprising:
- introducing an attB site into the genome of TIE-1;
- providing a suicide plasmid including an attP site and a target gene;
- introducing a φC31 integrase into the genome of TIE-1;
- and
- expressing the φC31 integrase in the presence of the attB site and the attP site such that the target gene is integrated into the genome.
A transgenic Rhodopseudomonas palustris TIE-1 (TIE-1) comprising at least one copy of RuBisCo integrated into a genome of TIE-1.
A transgenic Rhodopseudomonas palustris TIE-1 (TIE-1) comprising a gene cassette including phaJ, ter, and adhE2.
Dependencies are inferred from the claim text.