Site-specific antibody conjugates and the methods for preparation of the same
AssigneeGlyco-Therapy Biotechnology Co., Ltd.
InventorYi YANG
Life of this patent
Estimating the term…
Abstract
The present disclosure provides a site-specific protein conjugate and the method for preparation of the same. The protein conjugate comprising a protein and an oligosaccharide, wherein said oligosaccharide comprises wherein: said GlcNAc is directly or indirectly linked to an amino acid of said protein; said Gal is a galactose; said (Fuc) is a fucose, b is 0 or 1; said Fuc* comprises a fucose or a fucose derivative linked to a molecule of interest (MOI), said protein comprises an antigen binding fragment and/or a Fc fragment.
Claims226
A protein conjugate, comprising a protein and an oligosaccharide, wherein said oligosaccharide comprises a structure of
- wherein:
- said GlcNAc is directly or indirectly linked to an amino acid of said protein;
- said Gal is a galactose;
- said (Fuc) is a fucose, b is 0 or 1;
- said Fuc* comprises a fucose or a fucose derivative and a molecule of interest (MOI);
- said protein comprises an antigen binding fragment and/or a Fc fragment.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method for preparing a protein conjugate, comprising a step (a):
- contacting a fucose derivative donor Q-Fuc*′ to a protein comprising an oligosaccharide in the presence of a catalyst,
- wherein said oligosaccharide comprises -GlcNAc(Fuc)b-Gal, to obtain a protein conjugate comprising
- wherein:
- said GlcNAc is directly or indirectly linked to an amino acid of said protein;
- said Gal is a galactose;
- said (Fuc) is a fucose, b is 0 or 1;
- said Fuc*′ comprises a fucose or fucose derivative Fuc and a molecule of interest (MOI′);
- said protein comprises an antigen binding fragment and/or a Fc fragment;
- said Q-Fuc*′ is a molecule comprises said Fuc*′.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
The method of 87, wherein said GlcNAc in said oligosaccharide is directly linked to an Asn residue of said protein.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
The method of 87, wherein said MOP′ of Fuc* comprises an active moiety;
- wherein said active moiety of said MOP′ comprises a P, and P is a biologically and/or pharmaceutically active substance;
- wherein step (a) comprising contacting said Q-Fuc*′ with said protein to obtain a protein conjugate comprising
- wherein Q-Fuc*′ is Q-Fuc-(F)m-(L)n-P, Fuc is the fucose or fucose derivative of the Fuc*′, F is a connector, L is a linker, P is a biologically and/or pharmaceutically active substance, b is 0 or 1, m is 1, and n is 0 or 1;
- wherein said connector F is
- wherein said FL is a spacer and s is 0 or 1.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.