Compositions and methods for detecting picobirnavirus
OwnerMichael G. BERG, Kenn FORBERG +2inventors
InventorsMichael G. BERG, Kenn FORBERG, Todd V. MEYER, Ka-Cheung LUK
Life of this patent
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Abstract
Provided herein are compositions, methods, and kits for detecting human picobirnavims (PBV). In certain embodiments, provided herein are PBV specific nucleic acid probes and primers, and methods for detecting PBV nucleic acid.
Claims31
A primer for amplifying human picobirnavirus (PBV) in a sample, wherein the primer comprises a sequence with 80% or more sequence identity to SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or complements thereof.
A probe for detecting PBV in a sample, wherein the probe comprises a sequence with 80% or more sequence identity to SEQ ID NO:
- 6, SEQ ID NO:
- 9, or complements thereof.
A composition for amplifying PBV in a sample, comprising:
- a) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 4 or a complement thereof and at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 5 or a complement thereof;
- or
- b) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 7 or a complement thereof and at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 8 or a complement thereof.
A composition for detecting PBV in a sample, comprising
- a) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 4 or a complement thereof, at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 5 or a complement thereof, and a probe comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 6 or a complement thereof;
- or
- b) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 7 or a complement thereof, at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 8 or a complement thereof, and a probe comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 9 or a complement thereof.
A method of detecting PBV in a sample, comprising contacting the sample with at least one primer and/or at least one probe, wherein the PBV comprises at least one sequence selected from SEQ ID NO:
- 1, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11.
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A kit for detecting PBV in a sample, comprising:
- a) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 4 or a complement thereof, at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 5 or a complement thereof, and a probe comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 6 or a complement thereof;
- b) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 7 or a complement thereof, at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 8 or a complement thereof, and a probe comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 9 or a complement thereof;
- or
- c) at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 4 or a complement thereof, at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 5 or a complement thereof, and a probe comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 6 or a complement thereof, at least one forward primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 7 or a complement thereof, at least one reverse primer comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 8 or a complement thereof, and a probe comprising a sequence with 80% or more sequence identity to SEQ ID NO:
- 9 or a complement thereof.
An isolated polynucleotide having:
- (a) 50% or more sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 6, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or fragments thereof;
- (b) 80% or more sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 6, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or fragments thereof;
- (c) 90% or more sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 6, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or fragments thereof;
- or
- (d) 95% or more sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 6, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or fragments thereof.
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An isolated polypeptide having:
- (a) 80% or more sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 11, or fragments thereof;
- (b) 90% or more sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 11, or fragments thereof;
- or
- (c) 95% or more sequence identity to SEQ ID NO:
- 7, SEQ ID NO:
- 11, or fragments thereof.
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Dependencies are inferred from the claim text.