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US 2023/0148256 A1Published application
Expression of modified proteins in a peroxisome
AssigneesProvenance Bio, LLC, The Regents of the University of California
InventorsGenevieve Vidanes, John Dueber, Zev Gartner, Chirag Vazirani
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Abstract
Disclosed herein include methods and compositions for making proteins in peroxisomes as well as methods of making cells for producing proteins in peroxisomes. Also disclosed herein are cells for producing a protein in a peroxisome, and methods for producing a protein in a eukaryotic cell containing a peroxisome as described herein.
Claims34
A method of producing a modified protein in a peroxisome, the method comprising:
- providing a cell;
- introducing a first nucleic acid into the cell, wherein the first nucleic acid comprises a first sequence encoding a heterologous protein fused to a peroxisome-targeting sequence;
- and
- introducing a second nucleic acid into the cell, wherein the second nucleic acid comprises a second sequence encoding a heterologous modification enzyme fused to a peroxisome-targeting sequence.
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Dependencies are inferred from the claim text.