Enzyme for the conversion of chlorogenic acid into isochlorogenic acid

A protein comprising, or consisting of, an amino acid sequence selected from:

• SEQ ID NO:
• 1,
• a sequence having at least 80% identity with SEQ ID No. 1,
• a fragment including at least 50 amino acids of said SEQ ID No. 1 and
• a fragment including at least 50 amino acids of said sequence having at least 80% identity with SEQ ID No. 1,
• said protein being capable of converting chlorogenic acid into isochlorogenic acid.

A method for producing isochlorogenic acid comprising contacting, under appropriate reaction conditions, chlorogenic acid and a protein comprising, or consisting of, an amino acid sequence selected from:

• SEQ ID NO. 1,
• a sequence having at least 80% identity with SEQ ID No. 1,
• a fragment including at least 50 amino acids of said SEQ ID No. 1, and
• a fragment including at least 50 amino acids of said sequence having at least 80% identity with SEQ ID No. 1,
• said protein being capable of converting chlorogenic acid into isochlorogenic acid, to obtain an isochlorogenic acid-enriched composition.

A method of converting chlorogenic acid into isochlorogenic acid, comprising:

• adding to an extract comprising chlorogenic acid at least one protein, said protein comprising, or consisting of an amino acid sequence selected from:
• SEQ ID NO. 1,
• a sequence having at least 80% identity with SEQ ID No. 1
• a fragment including at least 50 amino acids of said SEQ ID No. 1 and
• a fragment including at least 50 amino acids of said sequence having at least 80% identity with SEQ ID No. 1,
• said protein being capable of converting chlorogenic acid into isochlorogenic acid, or a host cell expressing such a protein, to convert chlorogenic acid into isochlorogenic acid.