Regulatory nucleic acid molecules for enhancing gene expression in plants
AssigneeBASF SE
InventorsFrank Meulewaeter, Christophe Liseron-Monfils
Life of this patent
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Abstract
The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.
Claims17
A method for enhancing expression derived from a plant promoter comprising functionally linking to a promoter one or more nucleic acid expression enhancing nucleic acid (NEENA) molecule heterologous to said promoter comprising
- i) a nucleic acid molecule having the sequence of any one of SEQ ID NOs:
- 16 to 21, or
- ii) a nucleic acid molecule having a sequence with an identity of at least 90% to any one of SEQ ID NOs:
- 16 to 21, which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of any one of SEQ ID NOs:
- 16 to 21 or
- iii) a nucleic acid molecule hybridizing under stringent conditions to a nucleic acid molecule having a sequence of any one of SEQ ID NOs:
- 16 to 21, or
- iv) a fragment of 30 or more consecutive bases of a nucleic acid molecule of i) to iii) which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of any one of SEQ ID NOs:
- 16 to 21, or
- v) a nucleic acid molecule which is the complement or reverse complement of any of the previously mentioned nucleic acid molecules under i) to iv).
A recombinant expression construct comprising a NEENA molecule selected from the group of
- i) the nucleic acid molecule having a sequence of any one of SEQ ID NOs:
- 16 to 21, and
- ii) a nucleic acid molecule having a sequence with an identity of at least 90% to any one of SEQ ID NOs:
- 16 to 21, which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of any one of SEQ ID NOs:
- 16 to 21 and
- iii) a nucleic acid molecule hybridizing under stringent conditions to a nucleic acid molecule having a sequence of any one of SEQ ID NOs:
- 16 to 21, and
- iv) a fragment of 30 or more consecutive bases of a nucleic acid molecule of i) to iii) which has expression enhancing activity as the corresponding nucleic acid molecule having the sequence of any one of SEQ ID NOs:
- 16 to 21, and
- v) a nucleic acid molecule which is the complement or reverse complement of any of the previously mentioned nucleic acid molecules under i) to iv),
- functionally linked to one or more promoter and one or more expressed nucleic acid molecule wherein the promoter is heterologous to said one or more NEENA molecule.
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Dependencies are inferred from the claim text.