Indole diterpene biosynthesis

A host cell comprising a polypeptide selected from the group consisting of:

• a. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to the amino acid sequence of IdtA from Epichloë var. lolii strain AR37, or corresponding to a polypeptide encoded by the idtA gene from Epichloë festucae var. lolii strain AR37;
• b. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to the amino acid sequence of IdtD from Epichloë festucae var. lolii strain AR37, Epichloë festucae var. lolii strain AR127, Epichloë festucae var. lolii strain AR128, or Epichloë festucae var. lolii strain AR166, or corresponding to a polypeptide encoded by the idtD gene from Epichloë festucae var. lolii strain AR37, Epichloë festucae var. lolii strain AR127, Epichloë festucae var. lolii strain AR128, or Epichloë festucae var. lolii strain AR166;
• c. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to the amino acid sequence of IdtO from Epichloë festucae var. lolii strain AR37, or corresponding to a polypeptide encoded by the idtO gene from Epichloë festucae var. lolii strain AR37;
• d. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to the amino acid sequence of IdtF from Epichloë festucae var. lolii strain AR37 or Epichloë festucae var. lolii strain AR6, or corresponding to a polypeptide encoded by the idtF gene from Epichloë festucae var. lolii strain AR37 or Epichloë festucae var. lolii strain AR6;
• e. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to the amino acid sequence of IdtK from Epichloë festucae var. lolii strain AR37 or Epichloë festucae var. lolii strain AR6, or corresponding to a polypeptide encoded by the idtK gene from Epichloë festucae var. lolii strain AR37 or Epichloë festucae var. lolii strain AR6;
• f. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence set forth in any one of SEQ ID NO:
• 3, 6, 9, 17, 19, 21, 23, 50-53, or 70-74;
• or
• g. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to at least 10 contiguous amino acids from an amino acid sequence set forth in any one of SEQ ID NO:
• 3, 6, 9, 17, 19, 21, 23, 50-53, or 70-74;
• h. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to amino acid residues 150 to 239 of SEQ ID NO:
• 3;
• or
• i. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to at least 10 contiguous amino acids from an amino acid sequence set forth in amino acid residues 150 to 239 of SEQ ID NO:
• 3;
• j. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to amino acid residues 76 to 436 of SEQ ID NO:
• 6;
• or
• k. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to at least 10 contiguous amino acids from an amino acid sequence set forth in amino acid residues 76 to 436 of SEQ ID NO:
• 6;
• l. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to amino acid residues 39 to 174 of SEQ ID NO:
• 9;
• or
• m. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to at least 10 contiguous amino acids from an amino acid sequence set forth in amino acid residues 39 to 174 of SEQ ID NO:
• 9;
• n. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to amino acid residues 19 to 386 of SEQ ID NO:
• 17 or 19;
• or
• o. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to at least 10 contiguous amino acids from an amino acid sequence set forth in amino acid residues 19 to 386 of SEQ ID NO:
• 17 or 19;
• p. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to amino acid residues 353 to 487 of SEQ ID NO:
• 21 or 23;
• or
• q. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence corresponding to at least 10 contiguous amino acids from an amino acid sequence set forth in amino acid residues 353 to 487 of SEQ ID NO:
• 21 or 23;
• r. a polypeptide comprising, consisting essentially of, or consisting of an amino acid sequence encoded by a polynucleotide sequence set forth in any one of SEQ ID NO:
• 1, 2, 4, 5, 7, 8, 10 to 16, 18, 20, or 22;
• s. a polypeptide encoded by a polynucleotide sequence having at least about 90% nucleic acid sequence identity to a sequence set forth in any one of SEQ ID NO:
• 1, 2, 4, 5, 7, 8, 10 to 16, 18, 20, or 22;
• t. a polypeptide involved in the production of one or more janthitrem compound, including a janthitrem compound of formulae VI, or an epoxy-janthitrem compound of any one of formulae Ito III or V to VIII;
• u. a polypeptide comprising an enzymatic activity having as its substrate terpendole I or as its substrate or its product a compound of any one of formulae Ito VIII, such as a compound of any one of formulae IIA to IIE;
• v. a catalytically active fragment of any one of a) to u) above;
• and
• w. a polypeptide comprising, consisting essentially of, or consisting of a sequence of amino acid residues that has at least 70%, at least 75%, at least 80%, at least 85%, or at least 90% or greater amino acid sequence identity to any one of a) to t) above;
• or
• x. any combination of two or more of a) to w) above;
• wherein the polypeptide is heterologous to the cell or is heterologously expressed by the cell.