Back to results
US 2023/0141153 A1Published application
Stabilized rsv f proteins and uses thereof
AssigneeMerck Sharp & Dohme Corp.
InventorsLan Zhang, Arthur Fridman, Eberhard Durr, Andrew Bett
Life of this patent
Estimating the term…
Abstract
The disclosure relates to stable RSV F proteins and immunogenic compositions containing the same, as well as methods of using the immunogenic compositions and compositions comprising the RSV F proteins.
Claims17
A recombinant respiratory syncytial virus (RSV) F trimer, comprising:
- three recombinant RSV F peptides each comprising a deletion of wild type RSV F positions 98-146 and a linker of eight to fourteen amino acids between wild type RSV F positions 97 and 147, wherein the recombinant F peptides comprise the following modifications to stabilize the recombinant RSV F trimer in a prefusion conformations:
- (i) 190F and 207L amino acid substitutions,
- (ii) 155C and 290C amino acid substitutions, and one or more of (a)-(f) below
- (a) 486C and 490C amino acid substitutions;
- (b) 180C and 186C amino acid substitutions;
- (c) 486C and 489C amino acid substitutions;
- (d) 512C and 513C amino acid substitutions;
- (e) an 505C amino acid substitution;
- and
- (f) a deletion of RSV F wild type amino acids 482-513.
7 more elements
An RSV F peptide comprising:
- (i) a deletion of RSV F wild type amino acids at positions 98 to 146;
- (ii) a linker of eight to fourteen amino acids between RSV F wild type positions 97 to 147;
- (iii) 190F and 207L amino acid substitutions;
- (iv) 155C and 290C amino acid substitutions;
- and one or more of (a)-(f) below:
- (a) 486C and 490C amino acid substitutions;
- (b) 180C and 186C amino acid substitutions;
- (c) 486C and 489C amino acid substitutions;
- (d) 512C and 513C amino acid substitutions;
- and
- (e) an 505C amino acid substitution;
- (f) a deletion of RSV F wild type amino acids 482-513.
9 more elements
The immunogenic composition of 8 that is formulated with an adjuvant.
Dependencies are inferred from the claim text.