Recombinant microorganisms and uses therefor
AssigneeLanzaTech, Inc.
InventorsNicholas Alexander Fackler, Sean Dennis Simpson, Michael Koepke, Stephanie Rhianon Jones
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Abstract
The disclosure provides genetically engineered C1-fixing microorganisms capable of producing nanobodies. Additionally, the disclosure provides engineered microorganisms comprising one or more disrupted genes to strategically divert carbon flux away from nonessential or undesirable products towards products and/or co-products of interest. The disclosure enables co-production of useful chemicals from gaseous substrates.
Claims21
A genetically engineered C1-fixing microorganism capable of co-producing at least one target product and a nanobody comprising an exogenous nucleic acid encoding a nanobody gene, wherein the genetically engineered C1-fixing microorganism has improved carbon flux through acetoacetyl-CoA compared to a parental microorganism.
A genetically engineered C1-fixing microorganism capable of producing a nanobody comprising an exogenous nucleic acid encoding a nanobody gene.
A method of producing a nanobody and a co-product comprising:
- a) identifying at least one nanobody within a known variant library;
- b) engineering a C1-fixing microorganism capable of co-producing the nanobody and the at least one co-product, wherein the microorganism comprises an exogenous nucleic acid encoding a nanobody gene;
- and
- c) culturing the C1-fixing microorganism in the presence of a gaseous substrate comprising one or more of CO, CO2, and H2 to produce the nanobody and the co-product.
Dependencies are inferred from the claim text.