Engineered bacteria and methods of producing sustainable biomolecules
AssigneePresident and Fellows of Harvard College
InventorsShannon Noel NANGLE, Marika ZIESACK, Pamela A. SILVER, Sarabeth BUCKLEY
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Abstract
The technology described herein is directed to engineered chemoautotrophic bacteria and methods of producing sustainable biomolecules. In several aspects, described herein are engineered bacteria and corresponding methods, compositions, and systems for the production of products such as polyhydroxyalkanoates (PHA), sugar feedstocks, and lipochitooligosaccharide (LCO) fertilizers.
Claims72
An engineered Cupriavidus necator bacterium, comprising:
- at least one exogenous copy of at least one functional polyhydroxyalkanoate (PHA) synthase gene;
- and at least one exogenous copy of at least one functional thioesterase gene.
An engineered C. necator bacterium, comprising one or more of the following:
- a) at least one exogenous copy of at least one functional sugar synthesis gene;
- and/or
- b) at least one exogenous copy of at least one functional sugar porin gene.
An engineered heterotroph, comprising one or more of the following:
- a) at least one overexpressed functional sucrose catabolism gene;
- b) (i) at least one endogenous sucrose catabolism repressor gene comprising at least one engineered inactivating modification;
- or (ii) at least one exogenous inhibitor of an endogenous sucrose catabolism repressor gene or gene product;
- c) (i) at least one endogenous arabinose utilization gene comprising at least one engineered inactivating modification;
- or (ii) at least one exogenous inhibitor of an endogenous arabinose utilization gene or gene product;
- and/or
- d) at least one exogenous copy of at least one functional secondary product synthesis gene.
An engineered C. necator bacterium comprising at least one exogenous copy of at least one functional lipochitooligosaccharide synthesis gene.
Dependencies are inferred from the claim text.