Pei immobilized enzyme, and preparation method and use thereof

A preparation method for an immobilized enzyme, wherein the preparation method comprises:

• activating a cationic polymer with an activating agent, to obtain an activated cationic polymer;
• and
• enabling an enzyme to be immobilized with the activated cationic polymer, to obtain the immobilized enzyme;
• wherein, the activating agent is a glutaraldehyde modified or not modified by a polyol, or one or more cofactors;
• and
• the enzyme is selected from the group consisting of:
• a transaminase, a ketoreductase, a monooxygenase, an ammonia lyase, an ene reductase, an imine reductase, an amino acid dehydrogenase and a nitrilase.