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AssigneeThe Trustees of Indiana University
InventorsZhongchao Zhao, Adam Zlotnick
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Embodiments described herein provide orthohepadnavirus capsid protein (Cp) heterodimers, bicistronic vectors encoding the heterodimers, and methods for producing the heterodimers. The heterodimers can be used to form mosaic virus-like particles. In certain embodiments, the heterodimers can form a hexamer, which in turn can be used to nucleate capsid formation, resulting in a Janus particle-like virus-like particle. The hexamer's can then be removed, leaving holey capsids. The capsids can be loaded with, for example, one or more polypeptides, small molecules, or a combination of polypeptides and small molecules. The holes of the holey capsids can be filled with another orthohepadnavirus heterodimer or a homodimer.
An orthohepadnavirus capsid protein (Cp) heterodimer, comprising i) a first half-dimer comprising an orthohepadnavirus Cp, and ii) a second half-dimer comprising an orthohepadnavirus Cp, wherein the first half-dimer and the second half-dimer are not identical, wherein the first half-dimer and the second half-dimer are not linked by a peptide linker, and wherein the first half-dimer and the second half-dimer spontaneously dimerize with one another.
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Dependencies are inferred from the claim text.