Chimeric hiv virus-like particles
AssigneesArizona Board of Regents on behalf of Arizona State University, FruitVaccine Inc.
InventorsTsafrir S. Leket-Mor, Aigerim Kamzina, Hugh Mason, Indu Rupassara
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Abstract
Disclosed are compositions and virus-like particles (VLPs) self-assembled from the expression of human immunodeficiency virus (HIV) Gag protein and a fragment of gp41 protein containing its N-terminus ectodomain. The fragment of gp41 protein is linked to an antigen that is not a peptide or protein from HIV, which is presented by HIV VLP. In some aspects, the presented antigen is trimerized. Also disclosed are methods of inducing an immune response against the antigen.
Claims20
A human immunodeficiency virus (HIV) virus-like particle (VLP) comprising:
- a Gag protein;
- a fragment of gp41 protein comprising the C-terminal cytosolic domain of the gp41 protein;
- and
- an antigen,
- wherein the antigen is not a peptide or protein from HIV and is linked to the fragment of gp41 protein.
A replicating geminiviral expression system for producing a human immunodeficiency virus (HIV) virus-like particle (VLP) comprising:
- a first cloning vector with a T-DNA region comprising:
- a sequence encoding Rep;
- and
- a sequence encoding the promoter of ubiquitin-3 from potato with ubiquitin fusion;
- a second cloning vector with a T-DNA region comprising:
- a sequence encoding RepA;
- and
- a sequence encoding the promoter of ubiquitin-3 from potato with ubiquitin fusion;
- and
- a third cloning vector with a T-DNA region comprising an expression cassette and no replicon cassette, wherein the expression cassette comprises:
- a promoter region;
- a 5′ UTR;
- a sequence encoding Gag;
- a sequence encoding a fragment of gp41 and an antigen that is not a peptide or protein of HIV;
- and
- a 3′ UTR.
Dependencies are inferred from the claim text.