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US 2023/0074210 A1Published application
Methods for removal of adaptor dimers from nucleic acid sequencing preparations
AssigneeRoche Sequencing Solutions, Inc.
InventorsArkadiusz Bibillo, Randall W. Davis
Life of this patent
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Abstract
Sequencing adaptors and methods are provided for preparation of polynucleotides for sequencing. The sequencing adaptors contain a portion of a recognition sequence for a methyl-dependent endonuclease. Unwanted adaptor dimers that form during ligation of adaptors to target polynucleotides produce a complete restriction sequence and are cleaved by the endonuclease, followed by exonuclease digestion, thereby removing the dimers.
Claims9
1-11. (canceled)
An adaptor for polynucleotide sequencing, wherein the adaptor comprises:
- a double-stranded polynucleotide duplex region, wherein the double-stranded polynucleotide duplex region comprises a portion of a recognition sequence for a methyl-dependent endonuclease at its end, and wherein a complete recognition sequence for a methyl-dependent endonuclease will be formed if the duplex regions of two adaptors are covalently linked together to produce an adaptor dimer.
19-20. (canceled)
Dependencies are inferred from the claim text.