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US 2023/0069418 A1Published application
Method and antibody for detection of hbcag
AssigneesXiamen Innodx Biotech Co., Ltd, Xiamen University
InventorsZimin CHEN, Junhui XIONG, Jiaqi LIU, Shaojuan WANG, Shengxiang GE, Quan YUAN, Liuwei SONG, Xudong SUN
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Abstract
In the field of Hepatitis B virus (HBV) detection, disclosed are a method for detecting HBcAg by means of using a double antibody sandwich method, and an antibody and kit for detecting HBcAg; also included is a monoclonal antibody that can be used in the immunological detection of HBcAg in a tissue or cell sample.
Claims26
A kit, which comprises:
- (i) a first antibody, which is selected from an antibody or antigen-binding fragment thereof capable of specifically binding to an epitope contained in positions 150-183 of HBcAg protein;
- and,
- (ii) a second antibody, which is selected from an antibody or antigen-binding fragment thereof capable of specifically binding to an epitope contained in positions 141-154 of HBcAg protein.
A monoclonal antibody or antigen-binding fragment thereof capable of specifically binding to HBcAg, wherein,
- (i) the monoclonal antibody or antigen-binding fragment thereof comprises:
- a heavy chain variable region (VH) comprising the following 3 complementarity determining regions (CDRs):
- HCDR1 having a sequence set forth in SEQ ID NO:
- 11, HCDR2 having a sequence set forth in SEQ ID NO:
- 12, and HCDR3 having a sequence set forth in SEQ ID NO:
- 13;
- and/or, a light chain variable region (VL) comprising the following 3 complementarity determining regions (CDRs):
- LCDR1 having a sequence set forth in SEQ ID NO:
- 14, LCDR2 having a sequence set forth in SEQ ID NO:
- 15, and LCDR3 having a sequence set forth in SEQ ID NO:
- 16;
- or,
- (ii) an antibody or antigen-binding fragment thereof, which comprises:
- a heavy chain variable region (VH) comprising 3 CDRs contained in a heavy chain variable region set forth in SEQ ID NO:
- 9;
- and/or, a light chain variable region (VL) comprising 3 CDRs contained in a light chain variable region set forth in SEQ ID NO:
- 10;
- preferably, the 3 CDRs contained in the heavy chain variable region, and/or the 3 CDRs contained in the light chain variable region are defined by the Kabat, Chothia or IMGT numbering system;
- or,
- (iii) an antibody or antigen-binding fragment thereof, wherein the antibody is a monoclonal antibody produced by a hybridoma cell line 2A7 which is deposited in the China Center for Type Culture Collection (CCTCC) and has the deposit number of CCTCC NO. C2019302.
17 more elements
Dependencies are inferred from the claim text.