Insulin detection method and insulin detection kit

Abstract

An insulin detection method includes: adding a sample to an electrode having an insulin binding protein immobilized on a surface of the electrode, the insulin binding protein specifically recognizing insulin; and detecting an electrochemical change associated with formation of a complex of the insulin and the insulin binding protein. The insulin binding protein includes: a first region that includes an α-CT segment of an insulin receptor and does not include a β subunit of the insulin receptor, and a second region that includes an L1 domain of the insulin receptor and does not include the β subunit of the insulin receptor; and one of the first region and the second region is immobilized on the surface of the electrode.

Dependencies are inferred from the claim text.