Computer-implemented method, computer program product and system for simulating a cell culture process

A computer-implemented method for simulating a cell culture process, comprising:

• receiving one or more measurable parameter values relating to one or more operating conditions of the cell culture process;
• and
• simulating the cell culture process using:
• a model of the cell culture process;
• estimated values of unmeasurable parameters in the model;
• and
• the received one or more measurable parameter values,
• wherein the model of the cell culture process describes the cell culture process with coupled ordinary differential equations including measurable parameters and the unmeasurable parameters, one or more of the measurable parameters relating to said one or more operating conditions of the cell culture process;
• wherein one or more of the unmeasurable parameters relate to lysed cells in the cell culture process;
• and
• wherein the estimated values of the unmeasurable parameters are estimated using Bayesian inference with measurable parameter values that are measured with respect to at least one operation of the cell culture process.

A system for simulating a cell culture process, comprising:

• a storage medium storing a model of the cell culture process, the model describing the cell culture process with coupled ordinary differential equations including measurable parameters and unmeasurable parameters, wherein one or more of the measurable parameters relate to one or more operating conditions of the cell culture process and one or more of the unmeasurable parameters relate to lysed cells in the cell culture process;
• and
• a processor configured to:
• obtain measurable parameter values that are measured with respect to at least one operation of the cell culture process, the measurable parameter values being values of the measurable parameters in the model;
• estimate, using Bayesian inference with the obtained measurable parameter values, values of the unmeasurable parameters in the model;
• receive one or more new measurable parameter values relating to said one or more operating conditions of the cell culture process;
• simulate the cell culture process using:
• the model of the cell culture process;
• the estimated values of the unmeasurable parameters in the model;
• and
• the received one or more new measurable parameter values.