One-Step Sample Extraction Cassette And Method For Point-Of-Care Molecular Testing
AssigneeWisconsin Alumni Research Foundation
InventorsDavid Beebe, Duane Juang, Terry Juang
Life of this patent
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Abstract
A sample extraction cassette and method are provided to test for a target in a sample. The sample is obtained on a swab. The swab is inserted into a chamber in a case and into contact with a contact portion of a membrane. The contact portion of the membrane is axially moved into sequential communication with a wash fluid and a reaction fluid. The reaction fluid reacts with the target to provide a visual display corresponding to the presence of the target.
Claims36
A sample extraction cassette for point-of-care molecular testing for a target in a sample provided on a swab, comprising:
- a case having:
- a chamber configured for receiving the swab;
- a wash zone configured for receiving a wash fluid therein;
- a reaction zone configured for receiving a reaction fluid therein, the reaction fluid reacting with the target;
- and
- a membrane having a contact portion, the membrane slidably receivable into case and being moveable between:
- a transfer position wherein the contact portion of the membrane communicates with the sample provided on the swab when the swab is received in the chamber such that at least a portion of the sample is transferred to the contact portion;
- a wash position wherein the contact portion of the membrane is communicates with the wash zone;
- and
- a reaction position wherein the contact portion of the membrane communicates with the reaction zone.
A sample extraction cassette to test for a target in a sample provided on a swab, comprising:
- a case having:
- a chamber configured for receiving the swab;
- a wash zone configured for receiving a wash fluid therein;
- a reaction zone configured for receiving a reaction fluid therein, the reaction fluid reacting with the target;
- and
- a membrane slideable in a first direction within the case, the membrane having a contact portion which sequentially communicates with the swab, the wash zone and the reaction zone as the membrane is axially slid in the case in the first direction.
A method of point-of-care molecular testing for a target in a sample, comprising the steps:
- obtaining the sample on a swab;
- inserting the swab into a chamber in a case and into contact with a contact portion of a membrane;
- and
- axially moving the contact portion of the membrane into sequential communication with a wash fluid and a reaction fluid, the reaction fluid reacting with the target.
Dependencies are inferred from the claim text.