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US 2023/0067653 A1Published application
Engineered target specific nucleases
AssigneeSangamo Therapeutics, Inc.
InventorsRussell DeKelver, Ivan Krivega, Jeffrey C. Miller, Lei Zhang
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Abstract
Described herein are engineered nucleases comprising mutations in the cleavage domain (e.g., FokI or homologue thereof) and/or DNA binding domain (zinc finger protein, TALE, single guide RNA) such that on-target specificity is increased.
Claims21
1-35. (canceled)
An isolated polynucleotide encoding a Zinc Finger Nuclease (ZFN) and at least one of:
- (a) a polynucleotide encoding an exogenous peptide;
- (b) a modified 3′ UTR;
- and/or
- (c) a 5′ UTR.
1 more element
Dependencies are inferred from the claim text.