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US 2023/0067484 A1Published application
Methods and compositions for genomic integration
AssigneeMyeloid Therapeutics, Inc.
InventorsDaniel GETTS, Yuxiao Wang, Namita Bisaria, Inna Shcherbakova, Socheata Ly
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Abstract
Methods and composition for modulating a target genome and stable integration of a transgene of interest into the genome of a cell are disclosed.
Claims31
-30. (canceled)
A method of stably integrating a human insert sequence into genomic DNA of a target human cell, the method comprising:
- (a) contacting a composition to the target cell, the composition comprising an RNA molecule, wherein the RNA molecule comprises:
- (i) a human insert sequence, wherein the human insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous human therapeutic polypeptide, and
- (ii) a human mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the human mobile genetic element promotes integration of the human insert sequence into genomic DNA via target-primed reverse transcription (TPRT);
- and
- (b) stably integrating the human insert sequence into the genomic DNA of the target human cell;
- and
- (c) expressing the exogenous human therapeutic polypeptide in the target human cell.
4 more elements
Dependencies are inferred from the claim text.