Adaptors for nucleic acid constructs in transmembrane sequencing
AssigneeOxford Nanopore Technologies PLC
InventorBrian McKeown
Life of this patent
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Abstract
The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.
Claims29
An adaptor for sequencing nucleic acids, which comprises a region of double stranded nucleic acid, wherein at least one end of the region forms one half of a palindromic cleavage site and wherein the adaptor is differentially selectable from another adaptor.
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A pair of adaptors comprising a Type I adaptor and a Type II adaptor, wherein each type of adaptor in the pair is differentially selectable from the other type and wherein a complete palindromic cleavage site is formed if any combination of the two types of adaptor are ligated to one another,
- wherein the Type I adaptor comprises a region of double stranded nucleic acid, wherein at least one end of the region forms one half of a palindromic cleavage site and wherein the adaptor is differentially selectable from another adaptor, and the region is formed by hybridization between two separated regions of a single stranded nucleic acid and the adaptor comprises a hairpin loop;
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- wherein the Type II adaptor comprises a region of double stranded nucleic acid, wherein at least one end of the region forms one half of a palindromic cleavage site and wherein the adaptor is differentially selectable from another adaptor.
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Dependencies are inferred from the claim text.