Reagents for massively parallel nucleic acid sequencing

A method of analyzing a nucleic acid sequence, the method comprising:

• (a) bringing the nucleic acid sequence in contact with a fluorescently-labeled nucleotide conjugate and a polymerizing enzyme under conditions sufficient to form a binding complex comprising (i) the polymerizing enzyme and (ii) a nucleotide of the nucleic acid sequence bound to a nucleotide moiety of the fluorescently-labeled nucleotide conjugate, wherein the binding complex persists for a persistence time of 1 second or more before it is removed;
• (b) contacting the binding complex with an imaging reagent, wherein the imaging reagent comprises a photobleaching reducing agent;
• and
• (c) obtaining a fluorescent image of the binding complex in a presence of the imaging reagent.