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US 2023/0058852 A1Published application
Polymerizing enzymes for sequencing reactions
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Mohammad Wadud Bhuiya, Manjula Pandey, Jeremy Lackey, Jonathan M. Rothberg, Thomas Christian
Life of this patent
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Abstract
Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.
Claims20
-45. (canceled)
A recombinant polymerizing enzyme comprising a majority sequence from SEQ ID NO:
- 1, wherein the recombinant polymerizing enzyme comprises:
- a) a sequence that is at least 90% identical to amino acids 1-51 from SEQ ID NO:
- 2 in place of amino acids corresponding to amino acids 1-54 of SEQ ID NO:
- 1;
- b) a sequence that is at least 90% identical to amino acids 445-449 from SEQ ID NO:
- 5 in place of amino acids corresponding to amino acids 429-433 in SEQ ID NO:
- 1;
- c) a sequence that is at least 90% identical to amino acids 271-375 from SEQ ID NO:
- 5 in place of amino acids corresponding to amino acids 260-359 in SEQ ID NO:
- 1;
- and/or
- d) a sequence that is at least 90% identical to amino acids 72-89 from SEQ ID NO:
- 5 in place of amino acids corresponding to amino acids 75-91 in SEQ ID NO:
- 1.
11 more elements
A recombinant polymerizing enzyme having an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 1 and comprising:
- a) a sequence that is at least 90% identical to amino acids 1-51 from SEQ ID NO:
- 2 in place of amino acids corresponding to amino acids 1-54 of SEQ ID NO:
- 1;
- b) a sequence that is at least 90% identical to amino acids 445-449 from SEQ ID NO:
- 5 in place of amino acids corresponding to amino acids 429-433 in SEQ ID NO:
- 1;
- c) a sequence that is at least 90% identical to amino acids 271-375 from SEQ ID NO:
- 5 in place of amino acids corresponding to amino acids 260-359 in SEQ ID NO:
- 1;
- and/or
- d) a sequence that is at least 90% identical to amino acids 72-89 from SEQ ID NO:
- 5 in place of amino acids corresponding to amino acids 75-91 in SEQ ID NO:
- 1.
11 more elements
Dependencies are inferred from the claim text.