Fluorescence image analysis method, fluorescence image analyzer, fluorescence image analysis program

A fluorescence image analyzer comprising:

• an imaging unit for capturing, for a plurality of cells in which target sites on chromosomes are labeled with fluorescent dye, and the cells being contained in a sample, first images containing at least parts of the cells in the sample, and capturing second images containing fluorescence generated from the fluorescent dye labeling the target sites of the cells in the first images;
• and
• a processing unit;
• wherein the processing unit executes
• selecting a plurality of test cells to be tested and having specific morphological characteristics from the plurality of cells based on at least the first images;
• extracting bright spots due to the fluorescence generated from the fluorescent dye from at least a plurality of the test cells based on the second images;
• identifying cells with chromosomal abnormalities and/or cells without chromosomal abnormalities based on the extracted bright spots, and
• generating information related to a ratio of the cells with chromosomal abnormality to the test cells based on a number of the cells with chromosomal abnormality and/or a number of cells without chromosomal abnormality.

A fluorescence image analysis computer-executable program for executing

• obtaining, for a plurality of cells in which target sites on chromosomes are labeled with fluorescent dye, and the cells being contained in a sample, first images containing at least parts of the cells in the sample and second images containing fluorescence generated from the fluorescent dye labeling the target sites of the cells in the first image;
• selecting a plurality of test cells to be tested and having specific morphological characteristics from the plurality of cells based on at least the first images;
• extracting bright spots due to the fluorescence generated from the fluorescent dye from at least a plurality of the test cells based on the second images;
• identifying cells with chromosomal abnormality and/or cells without chromosomal abnormality based on the extracted bright spots;
• and
• generating information related to a ratio of cells with chromosomal abnormality to the test cells based on a number of cells with chromosomal abnormality and/or a number of cells without chromosomal abnormality.