Robust Protein Expression Enabled by Dynamic Control over Host Proteases

Abstract

Provide herein are engineered microbial strains having greatly improved expression of many challenging protein product. This system relies on controlled expression or dynamic reduction in activity of key housekeeping proteases. Dynamic control, implemented with CRISPR based gene silencing and or controlled protein degradation enables increased protein expression while minimizing the negative impact of complete deletions of these housekeeping enzymes.

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