High Fidelity SpCas9 Nucleases for Genome Modification
AssigneeSigma-Aldrich Co.
InventorFuqiang Chen
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Abstract
Engineered Cas9 protein variants and systems, nucleic acids encoding said protein variants and systems, and methods of making and using said protein variants and systems for genome modification.
Claims35
An engineered Streptococcus pyogenes Cas9 (SpCas9) protein variant comprising a mutation at two or more of amino acid positions 526, 562, 652, 661, 691, 780, 810, 848, 855, 1003, and 1060 (with reference to the numbering system of Streptococcus pyogenes Cas9, SpCas9), wherein a Lysine (K) at one or more of the foregoing amino acid positions is changed to a Leucine (L) or Glutamine (Q), and/or an Arginine (R) at one or more of the foregoing amino acid positions is changed to a Leucine (L) or Glutamine (Q).
A method for modifying the chromosomal sequence of a eukaryotic cell, the method comprising expressing in the eukaryotic cell the engineered SpCas9 protein variant of claim
Dependencies are inferred from the claim text.