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US 2023/0058264 A1Published application
Plasmid For Bacillus Expressing Fluorescent Reporter Genes
AssigneeNovozymes Bioag A/S
InventorsJared Heffron, John William Rice
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Abstract
The present invention relates to fluorescence reporter plasmid systems for enabling a Bacillus strain to fluoresce, and to methods for visualizing the state of Bacillus strains.
Claims18
A fluorescence reporter plasmid system for enabling a Bacillus strain to fluoresce, wherein the Bacillus strain fluoresces in its dormant endospore state and/or in its metabolically active vegetative state, and wherein the plasmid system is designed to function extra-chromosomally.
A method of visualizing the state of a Bacillus strain in an animal, a plant or a plant seed comprising:
- a) combining a Bacillus strain and a cell comprising a single or dual reporter plasmid system for enabling the Bacillus strain to fluoresce one fluorescent colour when in dormant endospore state and/or another fluorescent colour when in metabolically active vegetative state,
- b) treating an animal or a plant seed with the Bacillus strain of step (a),
- c) collecting samples from the treated animal or plant seed of step (b),
- d) optionally staining the samples of step (c) with DNA dyes, and
- e) imaging the samples of step (c) or (d) to visualize the the state of the Bacillus strain.
2 more elements
Dependencies are inferred from the claim text.