Solid reagent containment unit, in particular for a transportable microfluidic device for sample preparation and molecule analysis

A method, comprising:

• introducing a sample into an extraction chamber of a portable microfluidic device by introducing the sample into a sample inlet opening of the portable microfluidic device, the extraction chamber being defined by a first wall of the portable microfluidic device, a second wall of the portable microfluidic device, and a body of the portable microfluidic device that is between the first wall and the second wall;
• extracting molecules from the sample in the extraction chamber obtaining an analyzable sample;
• transferring the analyzable sample to an analysis chamber of the portable microfluidic device delimited by the body and the first wall;
• exposing the analyzable sample to a solid reagent in a reagent cavity of a reagent unit and on an integrated circuit chip of the reagent unit, the reagent cavity being delimited by a sidewall of an embossable holding material of the reagent unit and a surface of the integrated circuit chip;
• and
• analyzing the analyzable sample exposed to the solid reagent within the reagent cavity.

A method, comprising:

• introducing a sample into an extraction chamber of a portable microfluidic device dissolving a tablet present within the extraction chamber releasing a plurality of magnetic beads within the extraction chamber, the extraction chamber being defined by a first wall of the portable microfluidic device, a second wall of the portable microfluidic device, and a body of the portable microfluidic device that is between the first wall and the second wall;
• extracting molecules from the sample in the extraction chamber obtaining an analyzable sample including:
• introducing preparation reagents within the extraction chamber in which the sample is present;
• activating an anchor actuator to rotate an anchor within the extraction chamber to stir and mix the sample with the preparation reagents extracting the molecules from the sample and adhering the molecules to the magnetic beads;
• magnetically attracting the magnetic beads towards a blocking actuator by activating the blocking actuator to;
• and
• removing the preparation reagent spent from extracting the molecules from the sample;
• transferring the analyzable sample to an analysis chamber of the portable microfluidic device delimited by the body and the first wall;
• exposing the analyzable sample to a solid reagent in a reagent cavity of a reagent unit, the reagent cavity being delimited by a sidewall of an embossable holding material of the reagent unit and a surface of an integrated circuit chip of the reagent unit;
• and
• analyzing the analyzable sample exposed to the solid reagent within the reagent cavity.