Method for suppressing methylation of target dna in plant
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsGo ATSUMI, Takeshi MATSUMURA, Chikara MASUTA, Tsuyoshi INUKAI, Wataru MATSUNAGA, Reika ISODA
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Abstract
According to the present invention, methylation of a target DNA in a plant is suppressed quickly and easily without using recombinant technology, and a plant having desired traits is thereby created. In an RNA-directed DNA methylation mechanism, binding between scaffold RNA produced by transcription of the target DNA and an siRNA-AGO4 complex is inhibited.
Claims11
A method for inhibiting methylation of a target DNA in a plant cell, comprising a step of inhibiting binding of a scaffold RNA produced by transcription of the target DNA with an siRNA-AGO4 complex, in an RNA-directed DNA methylation mechanism.
An expression system for accumulation of a plant-derived functional component, comprising:
- (A) a plant body or a plant cell that produces a scaffold RNA produced by transcription of a DNA involved in synthesis or accumulation of the plant-derived functional component, and siRNA-AGO4 complex, in an RNA-directed DNA methylation mechanism, and
- (B) a short dummy RNA comprising a sequence complementary to the siRNA incorporated in the siRNA-AGO4 complex, or a sequence complementary to at least a portion of the scaffold RNA.
Dependencies are inferred from the claim text.