Methods for information transfer and related kits
AssigneeEncodia, Inc.
InventorsKevin L. GUNDERSON, Norihito MURANAKA
Life of this patent
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Abstract
The present disclosure relates to methods and kits for analyzing a macromolecule including information transfer between molecules, such as transfer of identifying information between nucleic acid molecules. In some embodiments, the macromolecule for analysis comprises a peptide, a polypeptide, or a protein. In some embodiments, the present disclosure relates to macromolecule analysis methods which employ barcoding and nucleic acid encoding of molecular recognition events, and/or detectable labels. Provided herein is a programmable system for information transfer comprising one or more adaptor molecules.
Claims23
A method for analyzing a macromolecule, comprising the steps of:
- (a) providing a macromolecule and an associated recording tag joined to a support;
- (b) contacting the macromolecule with a binding agent capable of binding to the macromolecule, wherein the binding agent comprises a coding tag with identifying information regarding the binding agent, to allow binding between the macromolecule and the binding agent;
- (c) providing an adaptor molecule comprising a first hybridization sequence and a secondary tag, wherein the first hybridization sequence is substantially complementary to at least a portion of the coding tag, to allow hybridization between the first hybridization sequence and the coding tag, wherein step (c) is performed before, after or simultaneously with step (b);
- (d) transferring information of the secondary tag to the recording tag to generate an extended recording tag, wherein the information of the secondary tag is transferred from the adaptor molecule to the recording tag after the coding tag associated with the binding agent hybridizes with the first hybridization sequence on the adaptor molecule;
- and
- (e) analyzing the extended recording tag.
(canceled)
15-16. (canceled)
A kit for analyzing a macromolecule, comprising:
- a binding agent comprising a coding tag, which comprises identifying information regarding the binding agent, wherein the binding agent is configured to bind to the macromolecule associated with a recording tag joined to a support;
- an adaptor molecule comprising a first hybridization sequence substantially complementary to at least a portion of the coding tag, and a secondary tag,
- wherein an information of the secondary tag is configured for transferring from the adaptor molecule to the recording tag to generate an extended recording tag after the coding tag associated with the binding agent hybridizes with the first hybridization sequence on the adaptor molecule;
- optionally, a means for transferring the information of the secondary tag or a means for analyzing the extended recording tag.
(canceled)
Dependencies are inferred from the claim text.