Thrombin Binding Circular Aptamer and Use Thereof

A thrombin binding circular aptamer, characterized in that, the thrombin binding circular aptamer has a nucleotide sequence of at least one selected from a) to e):

• a) a nucleotide sequence as shown in SEQ ID NO:1, wherein the 5′ end and 3′ end are connected to form a ring, the nucleotide sequence as shown in SEQ ID NO:1 being
• 5′-ATCTCGACTAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGT
• TGGCTGACAATACTCGTTTTTGGTGTCTCGGAT-3′;
• b) a nucleotide sequence obtained by substitution in the nucleotide sequence as shown in SEQ ID NO:1, the obtained nucleotide sequence being a circular DNA molecule capable of specific recognition of thrombin, wherein the nucleotide sequence obtained by substitution in the nucleotide sequence as shown in SEQ ID NO:1 has over 80% homology with the SEQ ID NO:1;
• c) a nucleotide sequence obtained by deletion in the nucleotide sequence as shown in SEQ ID NO:1, the obtained nucleotide sequence being a circular DNA molecule capable of specific recognition of thrombin, wherein the nucleotide sequence obtained by deletion in the nucleotide sequence as shown in SEQ ID NO:1 has over 70% homology with the nucleotide sequence as shown in SEQ ID NO:1;
• d) a nucleotide sequence obtained by adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO:1, the obtained nucleotide sequence being a circular DNA molecule capable of specific recognition of thrombin, wherein the nucleotide sequence obtained by adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID NO:1 has over 80% homology with the nucleotide sequence as shown in SEQ ID NO:1;
• e) a nucleotide sequence obtained by modification of the nucleotide sequence as shown in SEQ ID NO:1 with a chemical group.