Method for enhancing expression of insulin like growth factor 1 receptor in umbilical cord mesenchymal stem cell and method for obtaining multipotent umbilical cord mesenchymal stem cell

A method for obtaining a plurality of multipotent umbilical cord mesenchymal stem cells, the method comprising:

• providing a human umbilical cord tissue;
• cutting a Wharton’s jelly of the human umbilical cord tissue into pieces, then treating with collagenase type 1 and incubating for 14-18 hours;
• culturing explants in DMEM containing human cord blood serum (hUCS) and antibiotics for 5-7 days to allow for migration of a plurality of cells from the explants;
• and
• performing an isolation step, wherein the isolation step isolates the cells that express insulin-like growth factor 1 receptor (IGF1 R) and CD13, CD29, CD44, CD73, CD90, CD105, CD166, HLA-ABC, Oct-4, Sox-2, Nanog and SSEA-4 migrated from the explants to obtain the multipotent umbilical cord mesenchymal stem cells expressing IGF1 R and higher levels of Oct-4, Sox-2, Nanog and SSEA-4, which are representative markers for a multipotent capability.