Methods and compositions for making antibodies and antibody derivatives with reduced core fucosylation

A method for inhibiting the growth of cells expressing CD70 antigen, comprising administering to the cells a population of humanized anti-CD70 antibodies in an amount sufficient to inhibit cell growth, wherein the antibodies each comprise:

• (i) a humanized heavy chain comprising the three CDRs from SEQ ID NO:1 and a variable region framework sequence of human germline VH1-2 or VH1-18 and exon JH-6, provided that any of positions H46, H67, H68, H69, H70, H71, H80, H81, H82, H82A and H91 (Kabat numbering) can be occupied by the amino acid occupying the corresponding position from SEQ ID NO:1,
• (ii) a humanized light chain comprising the three CDRs from SEQ ID NO:2 and a variable region framework sequence of human germline VK exon B3 and JK exon Jk−1, provided that any of positions L25 and L33 can be occupied by the amino acid occupying the corresponding position from SEQ ID NO:2, and
• (iii) an Fc domain, wherein at least 50% of the antibodies in the population of antibodies lack core fucosylation.

A method for inhibiting the growth of cells expressing CD70 antigen, comprising administering to the cells a population of humanized anti-CD70 antibodies in an amount sufficient to inhibit cell growth, wherein the antibodies each comprise:

• (i) a humanized heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or amino acids 20-137 of SEQ ID NO:9,
• (ii) a humanized light chain variable region comprising the amino acid sequence of SEQ ID NO:10, and
• (iii) an Fc domain, wherein at least 50% of the antibodies in the population of antibodies lack core fucosylation.

A method for inhibiting the growth of cells expressing CD70 antigen, comprising administering to the cells a population of humanized anti-CD70 antibodies in an amount sufficient to inhibit cell growth, wherein at least 50% of the antibodies in the population of antibodies lack core fucosylation, and wherein the antibodies are h1F6.